weight marker thermo scientific page-ruler plus prestained protein ladder  (Thermo Fisher)

Journal: Scientific Reports

Article Title: Broadly reactive monoclonal antibodies against beta-lactamases for immunodetection of bacterial resistance to antibiotics

doi: 10.1038/s41598-025-04603-2

Figure Lengend Snippet: Production and analysis of the chimeric gp39m_linker_DHA protein. (a) Alignment of the conserved aa region of DHA-1 (GenBank no. AEP68014.1 ) (squared in yellow), CMY-34 (GenBank no. ABN51006.1 ), ACT-14 (GenBank no. AFU25647.1 ), PDC-195 (GenBank no. AHH52937.1 ) and ADC-144 (GenBank no. OVK75103.1 ) β-lactamases. An asterisk “*” denotes positions that have a single, fully conserved residue. A period “.” indicates conservation between groups of weakly similar properties. A colon “:” indicates conservation between groups of strongly similar properties. (b) Schematic representation of chimeric protein construction. A 77–93 aa region of DHA-1 (DHA-1 77−93 ) was fused with the tail tube protein gp39 of the bacteriophage, introducing a linker sequence (indicated in Supplementary information file 2, Fig. ). (c) SDS‒PAGE and WB analysis of yeast-expressed gp39 protein variants (indicated by yellow arrows). The lysates of yeast expressing gp39 and gp39m_linker_DHA were analysed. The dash “–” indicates the lysate of yeast transformed with the vector pFX7 without the DHA-1 77−93 coding sequence. For WB analysis, gp39 protein-specific in-house generated mouse polyclonal antibodies were used. M – molecular weight marker Page Ruler Prestained protein ladder (Thermo Scientific, 26616). The original blot and gel data are presented in Supplementary information file 2, Fig. . (d) Electron micrographs of chimeric nanotubes. The scale bars represent 200 nm. The original micrographs are presented in Supplementary information file 2, Fig. S3.

Article Snippet: M – molecular weight marker Page Ruler Prestained protein ladder (Thermo Scientific, 26616).

Techniques: Residue, Sequencing, Expressing, Transformation Assay, Plasmid Preparation, Generated, Molecular Weight, Marker

Journal: Scientific Reports

Article Title: Broadly reactive monoclonal antibodies against beta-lactamases for immunodetection of bacterial resistance to antibiotics

doi: 10.1038/s41598-025-04603-2

Figure Lengend Snippet: Representative reactivity patterns of the MAbs raised against the DHA-1 77−93 sequence with different β-lactamases. (a) MAb reactivity with recombinant β-lactamases in WB. Irrelevant rNDM-1 β-lactamase, gp39m_linker, maltose binding protein (MBP) and yeast lysate were used as negative controls. Original blots and gels are presented in Supplementary information file 2, Fig. S6. (b) MAb reactivity with the natural CMY-34 β-lactamase in C. portucalensis lysate tested by WB. E. coli BL21 lysate and MBP were used as negative controls. Original blots and gels are presented in Supplementary information file 2, Fig. S8. (c) Immunoprecipitation of rDHA-1 and natural CMY-34. C. portucalensis lysate was used as a source of natural CMY-34. E. coli BL21 lysate and protein dilution buffer (PBST) were used as negative controls. In-house produced irrelevant IgG3 MAb 20D1 against the SARS-CoV-2 spike protein was tested as an isotype control. The WB result was developed using horseradish peroxidase-conjugated MAb 26C9 against DHA-1 77−93 . SDS‒PAGE of immunoprecipitated proteins with MAbs 24F1, 21C12 and the isotype control are shown. Images of the original blots and gels are presented in Supplementary information file 2, Fig. S10. M – molecular weight marker PageRuler Prestained Protein Ladder (Thermo Scientific, 26616).

Article Snippet: M – molecular weight marker Page Ruler Prestained protein ladder (Thermo Scientific, 26616).

Techniques: Sequencing, Recombinant, Binding Assay, Immunoprecipitation, Produced, Control, Molecular Weight, Marker

Journal: iScience

Article Title: The human ABCG2 transporter engages three gates to control multidrug extrusion

doi: 10.1016/j.isci.2025.112125

Figure Lengend Snippet:

Article Snippet: Page Ruler Prestained Plus Protein Ladder , Thermo Fisher Scientific , Cat#26619.

Techniques: Recombinant, Modification, Cell Culture, Software, Sequencing

Journal: The EMBO Journal

Article Title: Maintenance of p-eIF2α levels by the eIF2B complex is vital for colorectal cancer

doi: 10.1038/s44318-025-00381-9

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Page Ruler Prestained Protein Ladder , Thermo Fisher Scientific , 26617.

Techniques: Recombinant, Sequencing, Protease Inhibitor, Viability Assay, Western Blot, SYBR Green Assay, Membrane, RNA Sequencing, Software, Imaging, Control, Real-time Polymerase Chain Reaction, High Content Screening